The germline is the bridge between one generation and the next. As such, the survival of sexually reproducing species is dependent on several intricate cellular programs including germline stem cells homeostasis and meiotic differentiation. In the Allard laboratory, we mobilize the cytological and genetics advantages of mammalian stem cells as well as the nematode to understand the pathways that are disrupted by environmental exposures and lead to germline dysfunction. Our projects under this theme are: (a) The comparative analysis of BPA and of the BPA substitutes (found in “BPA-free” products) on reproduction. (b) The examination of the interplay between cholesterol metabolism, BPA exposure and germline function. And (c) the development and application of a high-throughput screening for the identification of reproductive toxicants.